Regulatory

Part:BBa_K2123107:Design

Designed by: Maria Clara Tavares Astolfi   Group: iGEM16_UFAM-UEA_Brazil   (2016-10-13)


Universal promoter (Ptac + PJK26) in tandem + mer operator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 105
    Illegal EcoRI site found at 2
    Illegal XbaI site found at 17
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2
    Illegal SpeI site found at 106
    Illegal PstI site found at 120
    Illegal NotI site found at 8
    Illegal NotI site found at 113
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2
    Illegal BglII site found at 20
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2
    Illegal suffix found in sequence at 106
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2
    Illegal XbaI site found at 17
    Illegal SpeI site found at 106
    Illegal PstI site found at 120
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Synthesis


Source

Synthesis

References